ABSTRACT
Objective: To investigate the effect of baicalein (BAI) on the apoptosis of gastric cancer MGC-803 cells. Methods: After MGC-803 cells were treated with different concentrations of BAI, the proliferation and cell apoptosis of MGC-803 cells were detected by MTT and FCM assays, respectively. The cell apoptosis bodies formation of MGC-803 cells after treatment with different concentrations of BAI was observed by Hoechst 33342 staining, and the expression levels of the apoptosis-related proteins of MGC-803 cells were detected by Western blotting. Results: The 5, 10, 15, 25 and 50 mol/L BAI could inhibite the proliferation of gastric cancer MGC-803 cells (all P < 0.05). The apoptotic rate of gastric cancer MGC-803 cells after treatment with 10, 15 and 25 mol/L of BAI was increased (all P < 0.05). The number of apoptotic bodies in gastric cancer MGC-803 cells after treatment with 10, 15 and 25 mol/L of BAI was increased by Hoechst 33342 staining (all P < 0.05). The expression levels of cleaved-caspase-3 and cleaved-poly ADP-ribose polymerase (PARP) in MGC-803 cells were up-regulated after treatment with BAI, and the expression level of Bcl-2 was down-regulated (all P < 0.01). Conclusion: BAI can suppress the proliferation of gastric cancer MGC-803 cells, and induce the cell apoptosis. The mechanism may be related to the regulation of the expressions of the apoptosis-related proteins including caspase-3, PARP and Bcl-2 by BAI.
ABSTRACT
Objective To evaluate the clinical effect of laparoscope and open surgery for treatment of gynecological diseases. Methods 620 female patients with gynecological diseases were divided into two groups randomly (Group A: n = 310, Group B: n = 310). Group A was treated by laparoscopic surgery, Group B was treated by open abdominal surgery. The clinical effects of laparoscope and open abdominal surgery were compared between the two groups. Results Group A had better results in intraoperative blood loss, recovery time of intestine and stomach function, get out of bed time and hospitalization time than that in group B, there were significant difference (P 0.05). Conclusions Laparoscopic surgery has better effects for treatment of gynecological diseases than open abdominal surgery.